The auther investigated the minimal inhibitory concentration (MIC) of some antifungal agents against Genus Pityrosporum.
The tested strains were a total of 15 in number. They were 3 standard strains of Pityrosporum (P. orbiculare ATCC42132, P. ovale ATCC14521, P. pachydermatis ATCC14522), 3 of P. orbiculare from patients with tinea versicolor, 3 of P. orbiculare
from
seborrheic dermatitis, 3 of P. orbiculare and of P. ovale from normal individuals, The tested antifungal agents were amphotericin-B, ketoconazole, itraconazole, clotrimazole, sodium thiosulfate, selenium sulfide. A various concentration of drugs
were
mixed into media containing distilled water, neopeptone, agar, dextrose, yeast extract, olive oil, and Tween 80. The inoculum contained 20,000-40,000 colony forming units(cfu) of Pityrosporum.
@ES The results were follows:
@EN 1. MIC ranges of systemic amphotericin-B, ketoconazole and itraconazole were 0.01-25.6 g/ml, 0.01-3.2 g/ml and 0.05-1.6 g/ml, respectively. Itraconazole showed the lowest value.
2. MIC ranges of topical clotrimazole, sodium thiosulfate and sulfide were 0.2-320 g/ml, 1-200mg/ml and 0.02-0.64mg/ml, respectively, Ketoconazole cream showed the lowest value.
3. The lowest values of MIC of antifungal agents against. P. orbiculare were 0.05-1.6 g/ml for systemic intraconazole, 0.2-320 g/ml for clotrimazole. Those aginst. P. ovale were 0.1-0.2 g/ml for itraconzole, 0.02-0.04mg/ml for selenium sulfide.
P.
ovale, as a whole, was more sensitive than P. orbiculare.
4. The lowest values of MIC against Pityrosporum for the lesions of tinea versicolor were 0.2-1.6 g/ml for intraconazole, 2.5-320 g/ml for clotrimazole, 0.02-0.32mg/ml for selenium sulfide. Those against Pityrosporum from the lesions of
seborrheic
dermatitis were 0.2-0.4 g/ml for intraconazole, 0.2-5 g/ml for clotrimazole, Pityrosporum from the lesions of seborrheic dermatitis, as a whole, was more sensitive than Pityrosporum from tinea versicolor.
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